bsa pbstr Search Results


hrp conjugated streptavidin  (Thermo Fisher)
99
Thermo Fisher hrp conjugated streptavidin
Hrp Conjugated Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase conjugated to goat anti human igg antibody  (Jackson Immuno)
96
Jackson Immuno horseradish peroxidase conjugated to goat anti human igg antibody
Horseradish Peroxidase Conjugated To Goat Anti Human Igg Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs t primary antibodies against p p38  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology pbs t primary antibodies against p p38
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Pbs T Primary Antibodies Against P P38, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t primary antibodies against p p38/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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goat antimouse hrp antibody  (Bio-Rad)
99
Bio-Rad goat antimouse hrp antibody
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Goat Antimouse Hrp Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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bovine serum albumin (pbst/bsa)  (Boehringer Ingelheim)
90
Boehringer Ingelheim bovine serum albumin (pbst/bsa)
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Bovine Serum Albumin (Pbst/Bsa), supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine serum albumin (pbst/bsa)/product/Boehringer Ingelheim
Average 90 stars, based on 1 article reviews
bovine serum albumin (pbst/bsa) - by Bioz Stars, 2026-03
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extravidin peroxidase  (Millipore)
90
Millipore extravidin peroxidase
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Extravidin Peroxidase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/extravidin peroxidase/product/Millipore
Average 90 stars, based on 1 article reviews
extravidin peroxidase - by Bioz Stars, 2026-03
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bsa pbs t  (Thermo Fisher)
86
Thermo Fisher bsa pbs t
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Bsa Pbs T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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donkey anti mouse igg hrp  (Jackson Immuno)
96
Jackson Immuno donkey anti mouse igg hrp
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Donkey Anti Mouse Igg Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase hrp conjugated rabbit anti bovine igg antibody  (Jackson Immuno)
93
Jackson Immuno horseradish peroxidase hrp conjugated rabbit anti bovine igg antibody
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Horseradish Peroxidase Hrp Conjugated Rabbit Anti Bovine Igg Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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antibody against mouse ig fitc-conjugated  (Becton Dickinson)
90
Becton Dickinson antibody against mouse ig fitc-conjugated
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Antibody Against Mouse Ig Fitc Conjugated, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v v bsa pbst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc v v bsa pbst
Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of <t>Phospho-p38</t> and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
V V Bsa Pbst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of Phospho-p38 and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Journal: ACS applied materials & interfaces

Article Title: Tailored Mesoporous Silica Nanoparticles and the Chick Chorioallantoic Membrane: A Promising Strategy and Model for Efficient Blood-Brain Barrier Crossing.

doi: 10.1021/acsami.5c05429

Figure Lengend Snippet: Figure 4. In vitro Dox loading, release, and cytotoxicity in U87 Cells treated with Dox-loaded MSNs and transport of Dox across the BBB in the chick CAM. (a) Loading efficiency and loading amount of Dox-loaded MSNs. (b) Cumulative release profile of Dox from MSMs at pH 7.4 and 5.5. (c) Cytotoxicity analysis of U87 cells treated with Dox alone, MSNs (at equivalent Dox-loaded MSN concentration), and Dox-loaded MSNs (at equivalent Dox alone concentration) across various concentrations (1−20 μg/mL). Cell viability was measured using a CCK-8 assay 24 h after treatment. (d) Western blot analysis showing protein expression levels of Phospho-p38 and HMGB1. α-Tubulin was used as a loading control. (e) IVIS imaging of Dox distribution. (f) Radiant efficiency of Dox in chick embryonic brains. N = 10. (g) Quantitative analysis of Dox distribution in brain sections using TissueQuest software. Quantitative analysis of tissue staining and identification of nucleus-positive events were performed using TissueFAXS and TissueQuest software platforms, respectively (red: Dox; blue: DAPI-stained nuclei). (h) Confocal microscopy images of brain sections showing Dox distribution (red) and nuclei (blue, DAPI staining). Scale bar: 20 μm. (i) Drug permeability assay. Quantification of Dox content in brain homogenates using fluorescence spectrophotometry. Data are presented as mean ± SD. Statistical significance was analyzed using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: The PVDF membrane was blocked in PBS containing 0.1% Tween 20 (PBS-T) with 5% (w/v) BSA for 1 h and then washed three times with PBS-T. Primary antibodies against p-p38 (sc-17852-R, Santa Cruz Biotechnology, USA), HMGB1 (ab227168, abcam, UK), and αtubulin (sc-5286, Santa Cruz Biotechnology, USA) were incubated with the PVDF membrane at 4 °C overnight with gentle rocking.

Techniques: In Vitro, Concentration Assay, CCK-8 Assay, Western Blot, Expressing, Control, Imaging, Software, Staining, Confocal Microscopy, Permeability, Fluorescence, Spectrophotometry